Part:BBa_K3039005:Design

BHETase 1

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 958
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 958
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 958
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 958
  Illegal NgoMIV site found at 297
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The amino acid sequence was submitted to Twist Bioscience who codon optimised the sequence for E. coli, ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for potential TypeIIS assembly.

Source

Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by TWIST biosciences.